Summary information and primary citation
- PDB-id
-
6d8l;
DSSR-derived features in text and
JSON formats
- Class
- RNA
- Method
- X-ray (3.14 Å)
- Summary
- Group i self-splicing intron p4-p6 domain mutant u131a
(without isopropanol soaking)
- Reference
-
Shoffner GM, Wang R, Podell E, Cech TR, Guo F (2018):
"In
Crystallo Selection to Establish New RNA Crystal
Contacts." Structure, 26,
1275. doi: 10.1016/j.str.2018.05.005.
- Abstract
- Crystallography is a major technique for determining
large RNA structures. Obtaining diffraction-quality
crystals has been the bottleneck. Although several RNA
crystallization methods have been developed, the field
strongly needs additional approaches. Here we invented an
in crystallo selection strategy for identifying mutations
that enhance a target RNA's crystallizability. The strategy
includes constructing an RNA pool containing random
mutations, obtaining crystals, and amplifying the sequences
enriched by crystallization. We demonstrated a
proof-of-principle application to the P4-P6 domain from the
Tetrahymena ribozyme. We further determined the structures
of four selected mutants. All four establish new crystal
lattice contacts while maintaining the native structure.
Three mutants achieve this by relocating bulges and one by
making a helix more flexible. In crystallo selection
provides opportunities to improve crystals of RNAs or
RNA-ligand complexes. Our results also suggest that mutants
may be rationally designed for crystallization by "walking"
a bulge along the RNA chain.
