Summary information and primary citation
- PDB-id
-
3a6p;
DSSR-derived features in text and
JSON formats
- Class
- protein transport-nuclear protein-RNA
- Method
- X-ray (2.92 Å)
- Summary
- Crystal structure of exportin-5:rangtp:pre-mirna
complex
- Reference
-
Okada C, Yamashita E, Lee SJ, Shibata S, Katahira J,
Nakagawa A, Yoneda Y, Tsukihara T (2009): "A
high-resolution structure of the pre-microRNA nuclear
export machinery." Science,
326, 1275-1279. doi: 10.1126/science.1178705.
- Abstract
- Nuclear export of microRNAs (miRNAs) by exportin-5
(Exp-5) is an essential step in miRNA biogenesis. Here, we
present the 2.9 angstrom structure of the pre-miRNA nuclear
export machinery formed by pre-miRNA complexed with Exp-5
and a guanine triphosphate (GTP)-bound form of the small
nuclear guanine triphosphatase (GTPase) Ran (RanGTP). The
x-ray structure shows that Exp-5:RanGTP recognizes the
2-nucleotide 3' overhang structure and the double-stranded
stem of the pre-miRNA. Exp-5:RanGTP shields the pre-miRNA
stem from degradation in a baseball mitt-like structure
where it is held by broadly distributed weak interactions,
whereas a tunnel-like structure of Exp-5 interacts strongly
with the 2-nucleotide 3' overhang through hydrogen bonds
and ionic interactions. RNA recognition by Exp-5:RanGTP
does not depend on RNA sequence, implying that Exp-5:RanGTP
can recognize a variety of pre-miRNAs.
