Summary information and primary citation
- PDB-id
-
9i62;
DSSR-derived features in text and
JSON formats
- Class
- DNA binding protein
- Method
- cryo-EM (2.64 Å)
- Summary
- Cryoem structure of a rad51 d-loop
- Reference
-
Joudeh L, Appleby RE, Maman JD, Pellegrini L (2025):
"Structural
mechanism of strand exchange by the RAD51 filament."
Elife, 14. doi: 10.7554/eLife.107114.
- Abstract
- Homologous recombination (HR) preserves genomic
stability by repairing double-strand DNA breaks and
ensuring efficient DNA replication. Central to HR is the
strand-exchange reaction taking place within the
three-stranded synapsis wherein a RAD51 nucleoprotein
filament binds to a donor DNA. Here, we present the cryoEM
structure of a displacement loop of human RAD51 that
captures the synaptic state when the filament has become
tightly bound to the donor DNA. The structure elucidates
the mechanism of strand exchange by RAD51, including the
filament engagement with the donor DNA, the strand invasion
and pairing with the complementary sequence of the donor
DNA, the capture of the non-complementary strand and the
polarity of the strand-exchange reaction. Our findings
provide fundamental mechanistic insights into the
biochemical reaction of eukaryotic HR.
