Summary information and primary citation
- PDB-id
-
5wzk;
DSSR-derived features in text and
JSON formats
- Class
- RNA binding protein-RNA
- Method
- X-ray (2.8 Å)
- Summary
- Structure of
apum23-deletion-of-insert-region-ggaauugacgg
- Reference
-
Bao H, Wang N, Wang C, Jiang Y, Liu J, Xu L, Wu J, Shi Y
(2017): "Structural
basis for the specific recognition of 18S rRNA by
APUM23." Nucleic Acids Res.,
45, 12005-12014. doi: 10.1093/nar/gkx872.
- Abstract
- PUF (Pumilio/fem-3 mRNA binding factor) proteins, a
conserved family of RNA-binding proteins, recognize
specific single-strand RNA targets in a specific modular
way. Although plants have a greater number of PUF protein
members than do animal and fungal systems, they have been
the subject of fewer structural and functional
investigations. The aim of this study was to elucidate the
involvement of APUM23, a nucleolar PUF protein in the plant
Arabidopsis, in pre-rRNA processing. APUM23 is distinct
from classical PUF family proteins, which are located in
the cytoplasm and bind to 3'UTRs of mRNA to modulate mRNA
expression and localization. We found that the complete RNA
target sequence of APUM23 comprises 11 nt in 18S rRNA at
positions 1141-1151. The complex structure shows that
APUM23 has 10 PUF repeats; it assembles into a C-shape,
with an insertion located within the inner concave surface.
We found several different RNA recognition features. A
notable structural feature of APUM23 is an insertion in the
third PUF repeat that participates in nucleotide
recognition and maintains the correct conformation of the
target RNA. Our findings elucidate the mechanism for
APUM23's-specific recognition of 18S rRNA.
