Summary information and primary citation
- PDB-id
-
5c9h;
DSSR-derived features in text and
JSON formats
- Class
- RNA binding protein-RNA
- Method
- X-ray (3.0 Å)
- Summary
- Structural basis of template boundary definition in
tetrahymena telomerase
- Reference
-
Jansson LI, Akiyama BM, Ooms A, Lu C, Rubin SM, Stone MD
(2015): "Structural
basis of template-boundary definition in Tetrahymena
telomerase." Nat.Struct.Mol.Biol.,
22, 883-888. doi: 10.1038/nsmb.3101.
- Abstract
- Telomerase is required to maintain repetitive G-rich
telomeric DNA sequences at chromosome ends. To do so, the
telomerase reverse transcriptase (TERT) subunit
reiteratively uses a small region of the integral
telomerase RNA (TER) as a template. An essential feature of
telomerase catalysis is the strict definition of the
template boundary to determine the precise TER nucleotides
to be reverse transcribed by TERT. We report the 3-Å
crystal structure of the Tetrahymena TERT RNA-binding
domain (tTRBD) bound to the template boundary element (TBE)
of TER. tTRBD is wedged into the base of the TBE RNA
stem-loop, and each of the flanking RNA strands wraps
around opposite sides of the protein domain. The structure
illustrates how the tTRBD establishes the template boundary
by positioning the TBE at the correct distance from the
TERT active site to prohibit copying of nontemplate
nucleotides.
