Summary information and primary citation
- PDB-id
-
4nig;
DSSR-derived features in text and
JSON formats
- Class
- oxidoreductase-DNA
- Method
- X-ray (1.52 Å)
- Summary
- Crystal structure of alkb d135i-e136h mutant protein
with cofactors bound to dsDNA containing m6a-a
- Reference
-
Zhu C, Yi C (2014): "Switching
Demethylation Activities between AlkB Family RNA/DNA
Demethylases through Exchange of Active-Site
Residues." Angew.Chem.Int.Ed.Engl.,
53, 3659-3662. doi: 10.1002/anie.201310050.
- Abstract
- The AlkB family demethylases AlkB, FTO, and ALKBH5
recognize differentially methylated RNA/DNA substrates,
which results in their distinct biological roles. Here we
identify key active-site residues that contribute to their
substrate specificity. Swapping such active-site residues
between the demethylases leads to partially switched
demethylation activities. Combined evidence from X-ray
structures and enzyme kinetics suggests a role of the
active-site residues in substrate recognition. Such a
divergent active-site sequence may aid the design of
selective inhibitors that can discriminate these homologue
RNA/DNA demethylases.
