Summary information and primary citation
- PDB-id
-
3dw6;
DSSR-derived features in text and
JSON formats
- Class
- RNA
- Method
- X-ray (1.0 Å)
- Summary
- Crystal structure of the sarcin-ricin domain from e.
coli 23 s rrna, u2650-sech3 modified
- Reference
-
Olieric V, Rieder U, Lang K, Serganov A, Schulze-Briese
C, Micura R, Dumas P, Ennifar E (2009): "A fast
selenium derivatization strategy for crystallization and
phasing of RNA structures." Rna,
15, 707-715. doi: 10.1261/rna.1499309.
- Abstract
- Site-specific 2'-methylseleno RNA labeling is a
promising tool for tackling the phase problem in RNA
crystallography. We have developed an efficient strategy
for crystallization and structure determination of RNA and
RNA/protein complexes based on preliminary crystallization
screening of 2'-OCH(3)-modified RNA sequences, prior to the
replacement of 2'-OCH(3) groups with their 2'-SeCH(3)
counterparts. The method exploits the similar
crystallization properties of 2'-OCH(3)- and
2'-SeCH(3)-modified RNAs and has been successfully
validated for two test cases. In addition, our data show
that 2'-SeCH(3)-modified RNA have an increased resistance
to X-ray radiolysis in comparison with commonly used
5-halogen-modified RNA, which permits collection of
experimental electron density maps of remarkable
quality.
