Summary information and primary citation
- PDB-id
-
351d;
DSSR-derived features in text and
JSON formats
- Class
- DNA
- Method
- X-ray (1.64 Å)
- Summary
- X-ray crystal structures of the hexamer dcacgcg:
crystals grown in the presence of ruthenium (ii) hexammine
chloride
- Reference
-
Karthe P, Gautham N (1998): "Structure
of d(CACGCG).d(CGCGTG) in crystals grown in the presence
of ruthenium III hexammine chloride." Acta
Crystallogr.,Sect.D, 54, 501-509.
doi: 10.1107/S0907444997013954.
- Abstract
- Hexammine ions are strong inducers of the transition
from the B-form to the left-handed Z-form in DNA. Here the
structure of d(CACGCG). d(CGCGTG) obtained from crystals
grown from a drop containing [Ru(NH3)6]Cl3 is reported. The
structure is clearly characterized as Z-DNA. When compared
with the structure of d(CACGCG).d(CGCGTG)/MgCl2 and that of
d(CGCGCG)2, subtle differences are seen, most noticeably in
the water structure. Since stable well diffracting crystals
grow easily in the presence of [Ru(NH3)6]Cl3 and since this
ion is not visible in the electron density it is concluded
that the ion plays a non-specific role in stabilizing
Z-DNA.
